Kisspeptin-10 Peptide Powder

Kisspeptin-10 Peptide Powder, also called Metastin (45-54), is a 10 amino acid peptide that corresponds to the bioactive C-terminal 45-54 amino acids of metastin, a metastasis suppressor gene product. It acts as a potent agonist of GPR54 (OT7T175, AXOR12), with approximately 8-fold higher binding affinity than metastin (Ki values of 0.042 and 0.34 nM, respectively, for displacement of metastin (40-54)). Metastin (45-54) induces calcium mobilization in GPR54-transfected cells (EC50 = 0.18-1.1 nM). It inhibits the migration of GPR54-transfected CHO cells at concentrations of 10-100 nM, equaling the potency of full length metastin.

Shaanxi Medibridge Biotech Co., Ltd. supplies this material as research-grade powder or customized vial filling. Each batch can be supported with HPLC purity data, LC-MS identity testing, COA, and agreed packaging details. The product is supplied for research use only.

The kisspeptin (Kp) precursor contains 145 amino acids and can be hydrolyzed into Kp-54, Kp-14, Kp-13 and Kp-10. Kp-10, the shortest subtype with high activity, plays a vital role in many tissues. Kisspeptin 10 inhibited the expression of vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells, the main inducer of high vascular permeability (VP). Moreover, Kp-10 also promotes follicle maturation, ovulation and progesterone production. Exogenous Kp-10 injection decreased VP and VEGF by enhancing Kiss1r in OHSS rats without affecting ovulation.

Metastin serves as a tumor suppressor by hindering the growth of secondary tumors. When KISS1R (KISS1 receptor) is activated upon binding with KISS1, it boosts intracellular calcium levels and activates MAPKs, which in turn limit cell mobility and proliferation. Metastin is a potential therapeutic target for various cancers, including melanoma, thyroid, bladder, squamous cell carcinoma of the esophagus, gastric, hepatocellular, and breast cancers.

In addition to its role in cancer suppression, metastin plays a critical role in reproduction. It regulates the hypothalamic-pituitary-gonadal axis by modulating gonadotropin-releasing hormone (GnRH) secretion, which influences the onset of puberty. Gene mutations can lead to central precocious puberty (CPP) in males, while impaired metastin signaling can result in isolated hypogonadotropic hypogonadism (IHH) in both humans and mice. Metastin can also elevate plasma levels of gonadotropins (follicle-stimulating hormone and luteinizing hormone) and promote ovulation in prepubescent female rats.

Kisspeptin neurons are considered key upstream regulators of GnRH neurons. KP-10 can directly or indirectly stimulate GnRH neurons, causing them to release GnRH.

Subsequently:

KP-10 → KISS1R → GnRH ↑ → LH/FSH ↑ → Changes in gonadal steroid hormones

Therefore, KP-10 is commonly used to study:

• GnRH neuronal excitability
• LH pulse release
• Puberty initiation mechanisms
• Preovulatory LH surge
• Estrogen positive and negative feedback
• Hypothalamic ARC, AVPV/POA regional neural circuits

The study revealed that the down regulation of galactose and up regulation of glycogenic amino acids (GAAs), including L-alanine, L-glutamic acid, L-methionine, L-proline and L-valine, in the kisspeptin-10 group suggests the accelerated carbohydrate metabolism, which indicates a negative energy balance. Moreover, in the kisspeptin-10-treated rats, high levels of GAAs that were possibly derived from protein decomposition might cause a negative nitrogen balance. It was found that increases of ketogenic amino acids such as tyrosine indicate a disturbance in glucose use, which might be induced by kisspeptin-10 in this study. In contrast, cholesterol increased significantly in the rats treated with kisspeptin-10, suggesting disrupted lipid metabolism, which might result from a lipolysis blockade.

Fertility treatment and IVF adjacency

Kisspeptin has been explored to trigger oocyte maturation and time-sensitive LH surges in assisted reproduction - with interest in potentially lower OHSS risk profiles compared to some hCG triggers in selected study designs. Trial designs evolve; check current ClinicalTrials.gov entries rather than assuming one landmark result applies everywhere.

Hypogonadotropic hypogonadism (HH)

Because HH involves insufficient GnRH/LH/FSH signaling, kisspeptin's upstream role makes it a logical investigational tool for restoring gonadotropin pulses in research cohorts.

Puberty and reproductive axis diagnostics

Kisspeptin stimulation tests appear in endocrine diagnostics - measuring LH responses to assess hypothalamic–pituitary–gonadal (HPG) axis integrity.

Metabolic and non-reproductive angles (emerging)

Some literature explores kisspeptin signaling intersections with metabolism and energy balance. This is not the primary reason most peptide-community discussions mention KP-10, but it appears in broader neuroendocrine reviews.