Bromelain is a natural proteolytic enzyme complex mainly extracted from the stem and fruit of pineapple (Ananas comosus). It is usually obtained through a controlled process that includes juice extraction, filtration, ultrafiltration, and low-temperature drying, so the enzyme activity can be kept as much as possible. Unlike a single-compound ingredient, bromelain is made up of a group of cysteine proteases together with associated enzymes, forming a multi-component enzyme system with broad proteolytic activity.
In practical use, bromelain is valued for its ability to break down a wide range of protein substrates, which makes it useful in food processing, cosmetic formulations, and nutraceutical systems. Its enzymatic activity helps with protein hydrolysis, supports mild exfoliation in topical products, and is also often discussed in connection with inflammation-related and tissue-related pathways, so it works as a flexible functional enzyme ingredient across different formulation directions.
Shaanxi Medibridge Biotech Co., Ltd. focuses on supplying high-quality enzyme-based ingredients with stable activity standards and reliable supply support for different industrial and formulation needs.
COA
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Product Name |
CAS Number |
Batch Number |
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Pineapple Extract Bromelain Powder |
9001-00-7 |
MB2602061030 |
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Manufacturer Date |
Analysis Date |
Expiry Date |
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2026-02-06 |
2026-02-07 |
2028-02-05 |
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Sample Qty Base |
Packing |
Test Method |
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300KGS |
25kg/drum |
HPLC |
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Item |
Standard |
Results |
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Appearance |
Light yellow to yellow-brown amorphous powder |
Conforms |
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Identification |
Proteolytic enzyme reaction positive |
Conforms |
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Enzyme Activity |
1000–3000 GDU/g (as specified) |
2150 GDU/g |
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Solubility |
Soluble in water |
Conforms |
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Loss on Drying |
NMT 5.0% |
3.46% |
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Ash Content |
NMT 5.0% |
2.38% |
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Heavy Metals |
NMT 10 ppm |
Conforms |
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Arsenic (As) |
NMT 2 ppm |
Conforms |
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Microbial Limits |
Complies with food/nutraceutical standards |
Conforms |
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pH (1% Solution) |
5.0 – 7.0 |
6.1 |
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Bulk Density |
0.40 – 0.70 g/mL |
0.55 g/mL |
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Storage |
Store in cool & dry place. keep away from strong light and heat |
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Conclusion |
The batch conforms to the IN-HOUSE standard |
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Proteolytic Enzyme System
Bromelain works as a complex proteolytic enzyme system made up mainly of cysteine proteases from pineapple stem and fruit. It is not a single active molecule, but a combination of proteases and related components that work together to provide broad protein-degrading activity. This system can cleave peptide bonds in different kinds of protein substrates, including structural and globular proteins, under relatively mild conditions.
Its activity is usually expressed in GDU (Gelatin Digesting Units) or MCU (Milk Clotting Units), because for this kind of ingredient the key point is functional activity rather than conventional purity. Since bromelain is a multi-component enzyme system, it can show flexible performance across different pH environments and application systems, which is one reason it is widely used in formulations that need controlled enzyme activity and broad substrate compatibility.
Protein Hydrolysis & Bioactivity Mechanism
Bromelain shows effective proteolytic activity by breaking peptide bonds inside protein structures, which turns complex proteins into smaller peptides and amino acids. Its action is not highly substrate-specific, so it can work on different protein materials such as collagen, keratin, and other structural proteins under mild enzymatic conditions.
Besides direct protein hydrolysis, bromelain is also often discussed for broader bioactivity at the cellular level. It has been studied in connection with protein-mediated signaling pathways and processes related to cellular turnover and microenvironment balance. This combination of direct enzymatic breakdown and broader biological interaction supports its use in systems where both protein modification and functional enzyme activity are important.
Anti-Inflammatory & Circulatory Pathway
Bromelain is often associated with multi-pathway effects related to inflammation and circulation through its enzymatic activity. It has been studied for its influence on inflammatory mediators such as prostaglandins and for its interaction with pathways related to COX regulation, which is why it is often mentioned in connection with balanced inflammatory response.
It is also known for fibrinolytic activity, meaning it may help support the breakdown of fibrin and contribute to normal blood flow and microcirculation. Because of these combined properties, bromelain is often used as a functional enzyme ingredient in applications built around tissue recovery, fluid balance, and circulatory support.
Enzymatic Skin Renewal Model
Bromelain supports enzymatic skin renewal through its ability to hydrolyze protein components in the outermost layer of the skin. By breaking down keratin-based structures, it helps remove accumulated surface cells and supports a smoother, more refined skin texture.
Compared with mechanical exfoliation or acid-based peeling, this enzyme-driven process is generally seen as milder and easier to control, which makes it suitable for formulas aimed at gentle resurfacing. Its proteolytic action may also help improve ingredient penetration and overall skin-conditioning performance in topical products.
Physical Properties & Stability Profile
Bromelain is usually supplied as a light yellow to yellow-brown amorphous powder with a characteristic enzyme profile. It dissolves readily in water to form a clear to slightly opalescent solution, while remaining insoluble in most organic solvents such as ethanol and chloroform. As an enzyme complex, its quality is mainly defined by activity units like GDU or MCU rather than by purity percentage or molecular weight.
In terms of stability, bromelain can keep functional activity across a moderate pH range, but it is still sensitive to high temperature and extreme pH conditions, which may lead to denaturation and activity loss. Long exposure to heat, strong acids, or alkalis is better avoided. Proper handling under controlled conditions helps maintain stable performance in different formulation systems.
Application Scenarios
Bromelain is widely used in food processing, where its proteolytic activity helps with meat tenderization, protein hydrolysis, and beverage clarification. Its ability to break down complex proteins makes it a practical functional enzyme for improving texture and processing efficiency.
In cosmetic products, bromelain is used for enzymatic exfoliation and skin conditioning, helping improve skin texture and overall formula performance. It is also used in nutraceutical and dietary supplement products, where it works as a digestive enzyme and is often linked with broader physiological support.
Safety & Storage
Bromelain is generally considered suitable for food, cosmetic, and nutraceutical use when applied at appropriate levels. As a pineapple-derived proteolytic enzyme, it may still cause sensitivity or allergic reactions in some individuals, especially those with known pineapple allergies. During handling, it is better to avoid inhalation and direct contact with concentrated material.
For storage, the product should be kept in a tightly sealed container and protected from moisture, heat, and direct light. A cool and dry storage environment is usually recommended to help preserve enzyme activity and maintain long-term stability.
FAQ
Q: What does GDU mean in bromelain products?
A: GDU is a standard unit used to show the protein-digesting activity of bromelain.
Q: Can bromelain be used in cosmetic formulations?
A: Yes, it is commonly used in cosmetic products for gentle enzymatic exfoliation and skin conditioning.
Q: Is bromelain stable at high temperatures?
A: No, high temperature can reduce its enzyme activity.
Q: Can bromelain be combined with other active ingredients?
A: Yes, but compatibility should be checked, especially in systems with extreme pH or strong oxidizing ingredients.
Q: What is bromelain pineapple extract good for?
A: postoperative pain after wisdom tooth extraction, sinusitis, osteoarthritis, and exercise-induced muscle soreness
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